Revolve has developed a proprietary oligonucleotide-directed mutagenesis approach which allows for generating libraries of up to 1012 variants in a single-day single-tube reaction while eliminating wild-type and off-target mutations. Proprietary software for oligo design based on hybridization thermodynamics enables introduction of mutations with low bias, even distribution, and high library completeness. Libraries are constructed and delivered directly in the user's desired vector, avoiding the need for cloning and reduction in library diversity.
Pool of up to 1012 variants in desired vectors in 2 weeks
High completeness, even distribution and low bias compared to traditional methods.
Deep sequencing of a codon scanning library of a 287 codon gene
Comprehensive, expansive libraries lead to better solutions
Novel high-activity enzyme variants discovered from scanning and combinatorial libraries.
Complete fitness mapping of nearly all amino acid mutants of an enzyme using a scanning library.
Antibody/Binding Protein Engineering