High-Performance DNA Variant Libraries
Single pool plasmid libraries
>95% desired variants (<5% wildtype)
up to 1012 variants per library
2 week delivery
Combinatorial Libraries
Mutate multiple nearby or distal positions simultaneously
Choose any set of amino acids or codons
Enzyme Engineering
Optimize an enzyme active site
Alter substrate specificity and selectivity
Antibody/binder Engineering
Naïve CDR randomization
Affinity-maturation
Scanning and Random Libraries
Introduce one or multiple mutations per variant
Choose any set of amino acids or codons
Superior to error-prone PCR - access to all amino acids (100% vs. 32%)
Enzyme Engineering
Discover difficult-to-predict mutations that enhance stability and expression
Enhance stability and expression
Antibody/binder Engineering
Discover beneficial framework mutations
Optimize CDR loops
Fc engineering
Epitope mapping
Molecular biology
Map key promoter binding sites
Transcription factor engineering
Fluorescent protein engineering
Virus and protein evolution
Protein fitness landscapes
Alanine scanning
